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medeaLAB CASA (motility and morphology) - sperm analysis software for clinics, labs and breeders

medeaLAB CASA (computer aided sperm analysis): automatic analysis of sperm motility and morphology for assisted reproduction, veterinary research and toxicology

medeaLAB is an automated sperm motility analysis system for Windows. The medeaLAB system measures sperm concentration in ejaculate and performs the most objective characterization of sperm motility according to the WHO standard. Sperm tracks may be studied after the run as the tracks recorded in realtime are overlayed with the microscope image. All tracks are recorded to hard disk in a track file (FOW-file) together with patient data in a database record. The system hardware consists of standard components (PC, video camera, microscope). Advantages of our Sperm Motion Analysis System: Real-time tracking, recording to disk, long sequence recording, classification of motility pattern, details for each sperm motion track, editing of sperm tracks, morphology analysis, standard hardware components, simple maintenance System Description: Semen samples placed in standard counting chambers are observed with a analog or digital video camera connected to a C-mount equipped microscope. Also video tapes of spermatozoa may be used as input. With analog video input a framegrabber (videodigitizer) is necessary to digitize the video data. With a FireWire camera only a IEEE 1394 (FireWire) standard interface is necessary. Computer-assisted methods of sperm morphology evaluation Over the past 20 years, in order to avoid subjectivity, numerous studies that incorporate image analysis techniques in the assessment of sperm morphology have appeared. These techniques allow objective characterization of different sperm forms. The method of Moruzzi (1988) for quantification and classification of human sperm morphology by computer-assisted image analysis is semi-automated (25). Sperm heads are imaged through a microscope (NA=1.3) sampled at 0,125 um intervals and measured on an image analyzing system. Measurements included stain content, lenght, width, perimeter, area, and arithmetically derived combination. Additionally, each sperm image is optically sectioned at right angles to its major axis, to give a measure of lengthwise heterogeneity of shape. According to this method, the percentage of normal sperm heads can be accurately predicted using just four sperm head measurements. This classification procedure distinguishes normal from abnormal sperm with 95% accuracy and correctly assigned 86% of the sperm to one of 10 shape classes. Conclusions For clinicians responsible interpreting results to patients, it is important to emphasize that 0% strict criteria does not necessarily implicate that no normal sperm is present: it only signifies that no sperm meeting a set of criteria was observed in the sample. Nevertheless, even in cases of severe teratozoospermia fertilization may be possible; most promising is the development of intracytoplasmic sperm injection ( ICSI ) as the treatment of first choice in these cases.(27). Another conclusion is that only one standard method should be recommended for the preparation of morphology slides in order to ensure inter-laboratory comparability of results and to enhance the value of sperm morphology analysis for predicting fertility. At present there are five techniques of sperm preparation. For practical purposes, the Papanicolaou method seems the most suited, due to its widespread use. European Society of Human Reproduction and Society of Human Reproduction and Embryology (ESHRE) Embryology (ESHRE) Special Interest Group in Andrology Special Interest Group in Andrology Motility assessments morphological abnormalities, the Teratozoospermia Index (TZI) and how to calculate the Teratozoospermia Index (TZI) EQC, IQC Male Fertility Evaluation -------------------------------------------------------------------------------- The Biology of Male Reproduction Sperm cells are produced in the seminiferous tubules of the testes encased by large Sertoli cells which provide support for the meiotically dividing germ cells. The primary germ cell is the Spermatogonium which passes through a series of cell divisions to produce first primary spermatocytes, then secondary spermatocytes and finally spermatids. Each spermatid eventually matures into one spermatozoa (or sperm cell) during which process it sheds its cytoplasm which forms a droplet that travels down the length of the forming flagellum until it disappears in the mature sperm cell. Sperm cells in the testes are not yet capable of movement, they gain this ability as they pass through the epididymis in their maturation process. After passing through the cauda epididymis the maturing sperm cells enter the vas deferens which is a muscular duct which leads eventually to the urethra. The sperm in the vas deferens is mature and capable of motility. Sperm cells consist of a head which is made of a nucleus and an enzyme filled vesicle called the acrosome, a midpiece which contains mitochondria to power the flagellum, and a thin tailpiece. In immature sperm cells the cytoplasmic droplet may be seen at the junction of the midpiece and the tailpiece. This droplet is sometimes visible though not pronounced in rats, more pronounced in mice, and quite pronounced in hamsters. spermiogram sperm analysis teratozoospermia animal sperm morphology sperm images of sperm sperm motility counting mice amorphous sperm computer aided sperm analysis speed of sperm spermiogramm amorphous head casa sperm computer aided semen analyzer definition of sperm active motility normal sperm motility sperm generation sperm image sperm morphology changes sperm motility strict who criteria morphology amorphous head and morphology kruger criteria morphology sperm motility sperm abnormal sperm morphology amorphous head sperm amorphous sperms animal ivf criteria human semen evaluation diff-quick solution and ivf examination of human semen fertility motility human sperm video medealab casa morphology sperm species semen analysis sperm analyzer

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