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analysisClick one of the following links: sperm analysis motility morphology -------------------------------------------------------------------------------- Methods of Sperm Analysis Sperm can be analyzed with either manual or automated methods. The following descriptions are aimed more heavily at automated analysis since an automated system is capable of acquiring more information about sperm motility, especially progressive (forward moving) motility, than manual methods can. Automated analysis offers the advantages of being able to determine sperm velocity and linearity of the sperm track, and also is capable of saving the moving sperm image onto optical disk or some other medium for later retrieval and reanalysis. Prior to automated analysis it was not possible to independently verify the results of analysis at a later date. Manual motility analysis is accomplished by a trained observer looking at a sperm sample in either Test Yolk Buffer or Phosphate Buffered Saline and manually counting the number motile and non-motile then calculating the percent motility. Since the sperm counted are moving through the field and then quickly die it is not possible for a second observer to count the same sperm as the first observer. Generally two independent observers count seperate fields on seperate slides and an average is calculated from the two observations. Manual concentration analysis is conducted by producing a homogenate of standard volume from a cauda epididymal or testicular sample of known weight and counting the number of sperm heads in a known volume of homogenate using a hemocytmeter. Manual morphology analysis is conducted by analysis of Eosin Y stained sperm on fixed, coverslipped slides and manually counting incidences of morphological changes either to the head or tail of the sperm cells. Automated analysis can be performed by one of two systems available at the present time: The Computer Assisted Sperm Analyzer by medeaLAB spermanalysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker sperm_11 analysis_11 motility_11 morphology_11 computer aided sperm analysis: automatic analysis of sperm motility and morphology for assisted reproduction, veterinary research and toxicology sperm analysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker © |