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sperm motilityClick one of the following links: sperm analysis motility morphology -------------------------------------------------------------------------------- Acquiring the Images A good analysis requires attention to several factors that affect the quality of the image produced. First, the image must have sufficient clarity. This is why it is necessary to calibrate the phase annulus adjustment prior to each study. If the phase annulus is not properly adjusted a clear, bright image cannot be obtained. Also since the concentration calculation depends on the use of an accurate value for the screen area it is important to calibrate the magnification scale by use of a standard 100 micron grid, and to maintain consistency in the depth of chamber by using the same chamber for each concentration analysis. The UV filter block must also be in the proper position for the type of light source used and the proper objective lens must be in place. In order to insure a good motility sample it is important to insure that the slide stage temperature does not fluctuate out of range and that the sample on the slide is fresh. For this reason only five fields at a time can be acquired for motility analysis and for each set of fields the slide must be loaded with a fresh aliquot of sample preparation (A new software update has corrected the slide stage temperature update problem- check your version of the software to see if it updates the slide stage temperature during analysis scans). Care should be taken to choose the fields to acquire as quickly as possible so the sample will still be fresh during the scan. Fields must not be chosen based on activity of the sperm on the slide, but only based on factors which could make it difficult for the system to analyze the sample. Examples of reasons a field could be passed over during selection would be too high of a concentration of sperm, to low a concentration of sperm, excessive debris in the field, excessive "clumping" of sperm (heads stuck together), or bubbles. Non-motile sperm are NOT a valid reason to skip a field for analysis, though they are a reason to check for other factors that should be noted, such as out of range temperature or pH. – Sperm motility Sperm motility spermanalysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker sperm_11 analysis_11 motility_11 morphology_11 computer aided sperm analysis: automatic analysis of sperm motility and morphology for assisted reproduction, veterinary research and toxicology sperm analysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker © |