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parametersClick one of the following links: sperm analysis motility morphology The system described by Perez-Sanchez (1994) is based on the evaluation with a video digitizer board, a brightfield microscope with a 100x immersion objective, two monitors and the image analysis software (31)(Figure 6). Cells are displayed live on the video monitor and each sperm head image is processed automatically using a specific image analysis program for image enhancement and thresholding. Analysis of the sperm midpiece and tail is not included in the program. The system detects the boundary of the sperm head and the outline is displayed as white overlays superimposed on the video image. The set of morphometric parameters used by Perez constitutes a set of characteristics which is valid for characterization of the majority of morphological types of spermatozoa. Sample parameters: total number of spermatozoa, concentration, immobile, locally motile, rapidly motile spermatozoa, mean values for VCL, VSL, VAP, LIN, ALH, STR and WOB.Sperm morphology must only be considered as an indicator of fertilization potential, not as an absolute indicator of sterility. The aim should be to find the minimum sperm parameters associated with specific methods of seminal treatment and with culture techniques which can lead to a single, common strategy for male infertility problems. The Human Fertilization and Embryology Authority (1991) and WHO (1993) strongly suggest that for the treatment of infertility both partners must be evaluated in order to exclude subtle problems. sperm analysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker sperm_11 analysis_11 motility_11 morphology_11 computer aided sperm analysis: automatic analysis of sperm motility and morphology for assisted reproduction, veterinary research and toxicology sperm analysis motility morphology analyzer animal breeders clinics equine laboratory labs management reproductive research screens software species speed swine system toxicology assessment insemination program quality semen stallion veterinary acquisition automatic concentration data estimation image images measure parameters statistical video videotest windows accurate advanced analyzed assisted reproduction casa computer diagnostic fertility infertility laboratories male study database distribution motile movement spermatozoa IVF track spermiogram moving tracking WHO tracker © |